MUTATIONAL ANALYSIS OF THE SPECIFIC PRIMING SIGNAL ESSENTIAL FOR DNA-REPLICATION OF THE BROAD-HOST-RANGE PLASMID RSF1010

Authors
HONDA Y AKIOKA T TAKEBE S TANAKA K MIAO D HIGASHI A NAKAMURA T TAGUCHI Y SAKAI H KOMANO T BAGDASARIAN M
Citation
Y. Honda et al., MUTATIONAL ANALYSIS OF THE SPECIFIC PRIMING SIGNAL ESSENTIAL FOR DNA-REPLICATION OF THE BROAD-HOST-RANGE PLASMID RSF1010, FEBS letters, 324(1), 1993, pp. 67-70
Citations number
30
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
324
Issue
1
Year of publication
1993
Pages
67 - 70
Database
ISI
SICI code
0014-5793(1993)324:1<67:MAOTSP>2.0.ZU;2-3
Abstract
To analyze the RSF1010-specific priming mechanism, a library of random ly mutagenized ssiA sequences was constructed by chemical synthesis us ing mixed nucleotide phosphoramidites. Synthetic ssiA sequences with t he single base-substitutions were assayed for the SSI activity in E. c oli JM109 expressing RepB' primase. It was demonstrated that the activ ity of ssiA was damaged markedly by single base-substitutions within t he possible stem-loop structure and its 3'-flanking region. It is conc eivable that these domains are critical in recognition and primer synt hesis by RepB' primase.