DNA TYPING WITH DIGOXIGENIN-11-DIDEOXYURIDINETRIPHOSPHATE-LABELED OLIGONUCLEOTIDE PROBES ENABLES NONRADIOACTIVE ANALYSIS USING A DUAL DETECTION SYSTEM - APPLICATION FOR SCREENING HLA-DQA1 POLYMORPHISMS

Authors
RIHS HP THIELE A PERICHON B KRISHNAMOORTHY R BAUR X
Citation
Hp. Rihs et al., DNA TYPING WITH DIGOXIGENIN-11-DIDEOXYURIDINETRIPHOSPHATE-LABELED OLIGONUCLEOTIDE PROBES ENABLES NONRADIOACTIVE ANALYSIS USING A DUAL DETECTION SYSTEM - APPLICATION FOR SCREENING HLA-DQA1 POLYMORPHISMS, International archives of allergy and immunology, 101(1), 1993, pp. 7-12
Citations number
21
Categorie Soggetti
Allergy,Immunology
Journal title
International archives of allergy and immunology → ACNP
ISSN journal
10182438
Volume
101
Issue
1
Year of publication
1993
Pages
7 - 12
Database
ISI
SICI code
1018-2438(1993)101:1<7:DTWDO>2.0.ZU;2-E
Abstract
We describe a standardized, highly sensitive, nonradioactive detection procedure for HLA class-II typing of DQA1 alleles and suballeles whic h has important and cost-effective application in studying HLA disease associations. The procedure involves polymerase chain reaction-based target DNA sequence amplification and dot blotting followed by stringe nt hybridization with digoxigenin-11-dideoxyuridine triphosphate 3'-en d-labelled allele-specific oligonucleotide probes. The dual detection system described here makes this procedure very versatile.