EVIDENCE FOR AN ENHANCED TRANSCRIPTION-DEPENDENT DE-NOVO SYNTHESIS OFC EBP-BETA IN THE LPS ACTIVATION OF THE CHICKEN LYSOZYME GENE/

Authors
Citation
R. Goethe et L. Phivan, EVIDENCE FOR AN ENHANCED TRANSCRIPTION-DEPENDENT DE-NOVO SYNTHESIS OFC EBP-BETA IN THE LPS ACTIVATION OF THE CHICKEN LYSOZYME GENE/, Journal of leukocyte biology, 61(3), 1997, pp. 367-374
Citations number
43
Categorie Soggetti
Immunology,Hematology
ISSN journal
07415400
Volume
61
Issue
3
Year of publication
1997
Pages
367 - 374
Database
ISI
SICI code
0741-5400(1997)61:3<367:EFAETD>2.0.ZU;2-R
Abstract
C/EBP beta has been shown to mediate the lipopolysaccharide (LPS)-indu ced expression of the lysozyme gene through enhanced binding to the -6 .1-kb lysozyme enhancer. In this study, we describe the LPS regulation of the C/EBP beta gene in myelomonocytic HD11 cells. Northern analysi s showed that the steady state level of C/EBP beta mRNA increased in r esponse to LPS. The half life of C/EBP beta mRNA of about 1 h in HD11 cells was not affected by exposure to LPS, Nuclear run-on transcriptio n experiments with isolated nuclei revealed that the rate of C/EBP bet a gene transcription was enhanced by LPS, demonstrating that the C/EBP beta gene in BD11 cells was regulated at the transcriptional level in response to LPS, Furthermore, the LPS-induced binding activity of C/E BP beta to the -6.1-kb lysozyme enhancer was dependent not only on pro tein synthesis, but also on transcription. Thus, these results suggest ed that the LPS-induced binding activity to the -6,1-kb lysozyme enhan cer in HD11 cells was regulated by an enhanced transcription-dependent de novo synthesis of C/EBP beta.