ISOLATION AND CHARACTERIZATION OF A RHAMNOGALACTURONAN-II FROM RED WINE

Citation
T. Doco et Jm. Brillouet, ISOLATION AND CHARACTERIZATION OF A RHAMNOGALACTURONAN-II FROM RED WINE, Carbohydrate research, 243(2), 1993, pp. 333-343
Citations number
44
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
ISSN journal
00086215
Volume
243
Issue
2
Year of publication
1993
Pages
333 - 343
Database
ISI
SICI code
0008-6215(1993)243:2<333:IACOAR>2.0.ZU;2-S
Abstract
A complex polysaccharide originating from grape was purified from a re d wine by size-exclusion and ion-exchange chromatography. This polysac charide contained 2-O-methylfucose, rhamnose, fucose, 2-O-methylxylose , arabinose, apiose, galactose, galacturonic and glucuronic acids, and thiobarbituric acid assay-positive material, presumably 3-deoxy-D-man no-2-octulosonic acid (Kdo) and 3-deoxy-D-lyxo-2-heptulosaric acid (Dh a). Methylation structural analysis showed that this polysaccharide is a typical rhamnogalacturonan II (RG-II), a structurally complex pecti c polysaccharide that has been isolated previously from the primary ce ll walls of sycamore. Red wine RG-II presented discrete differences fr om previously described RG-II's, a greater richness in uronic acids an d some additional glycosyl linkages, which might be related to the pat tern of degradation of grape-wall pectic substances by endogenous pect inolytic enzymes after pressing the berries and subsequent fermentatio n to wine.