PLASMID PKKH - AN IMPROVED VECTOR WITH HIGHER COPY NUMBER FOR EXPRESSION OF FOREIGN GENES IN ESCHERICHIA-COLI

Authors
JING GZ HUANG Z LIU ZG ZOU Q
Citation
Gz. Jing et al., PLASMID PKKH - AN IMPROVED VECTOR WITH HIGHER COPY NUMBER FOR EXPRESSION OF FOREIGN GENES IN ESCHERICHIA-COLI, Biotechnology letters, 15(5), 1993, pp. 439-442
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
15
Issue
5
Year of publication
1993
Pages
439 - 442
Database
ISI
SICI code
0141-5492(1993)15:5<439:PP-AIV>2.0.ZU;2-J
Abstract
An improved vector of 2889 bp was constructed by mutation of ropy numb er control system, into which foreign genes without or with the start codon ATG can be directly inserted for high-level expression in Escher ichia coli. Deletion of the rop gene encoding a negative regulation pr otein ROP leads to increase the plasmid copy number, and finally makes the vector increase expression level more than 60% in comparison with initial one.