EXCYSTMENT INVITRO OF COTYLURUS-CORNUTUS (TREMATODA, STRIGEIDAE) METACERCARIAE

Authors
GRACZYK TK SHIFF CJ
Citation
Tk. Graczyk et Cj. Shiff, EXCYSTMENT INVITRO OF COTYLURUS-CORNUTUS (TREMATODA, STRIGEIDAE) METACERCARIAE, The Journal of parasitology, 79(3), 1993, pp. 448-450
Citations number
13
Categorie Soggetti
Parasitiology
Journal title
The Journal of parasitology → ACNP
ISSN journal
00223395
Volume
79
Issue
3
Year of publication
1993
Pages
448 - 450
Database
ISI
SICI code
0022-3395(1993)79:3<448:EIOC(S>2.0.ZU;2-W
Abstract
An acid-medium pretreatment appeared to be essential for in vitro excy station of Cotylurus cornutus metacercarial cysts. Both acid-pepsin or acid-saline were effective but optimum excystation occurred after 15 min acid-pepsin pretreatment and subsequent 2 hr treatment at 41.5 C i n an alkaline bile salt-trypsin medium. The rate of excystation was ra pid, reaching 78.1% by 1 hr and increasing to 84.3% after 2 hr. Storag e of metacercarial cysts up to 16 wk at 4 C in sterile Locke's 1:1 sol ution did not alter cyst viability.