RABBIT ANNEXINS - COMPARISON OF PROTEINS IN CELL-FREE HOMOGENATES ANDMEMBRANES OF VARIOUS TISSUES AND FAST PRELIMINARY IDENTIFICATION OF INDIVIDUAL COMPONENTS AFTER 2-DIMENSIONAL ELECTROPHORESIS

Annexins in cell-free homogenates and membranes of rabbit skeletal and heart muscles, liver, kidney, lungs, and brain have been compared by one- and two-dimensional electrophoresis. The pI and molecular weight values of the proteins have been determined Data on two-dimensional el ectrophoresis of annexins from different animals has been systematized Simple graphs are presented which allow identification of annexins on electrophoregrams. The technique is promising for identification of a dditional Ca2+-dependent membrane-binding proteins and can be used in the search for novel members of the annexin family.