Fifty-two Lactobacilli strains distributed in 9 species and 4 subspeci
es, and frequently found in breweries, have been tested by the API 50
CHL system and studied for their amino acid and base requirements on L
edesma agar, a rich synthetic medium. The data, sugar fermentation pro
files and nutritional profiles have been analysed by statistical metho
ds (multiple correspondence analysis (SPAD.N) and cluster analysis) Th
e results confirm a weak capacity to characterize Lactobacilli species
by the API system, however, the use of nutritional and fermentation p
rofiles permits discrimination between the various species