FLOW CYTOMETRIC DNA ANALYSIS AS PROGNOSTIC FACTOR IN HUMAN BREAST-CARCINOMA

Citation
L. Ottesdad et al., FLOW CYTOMETRIC DNA ANALYSIS AS PROGNOSTIC FACTOR IN HUMAN BREAST-CARCINOMA, Pathology research and practice, 189(4), 1993, pp. 405-410
Citations number
32
Categorie Soggetti
Pathology
ISSN journal
03440338
Volume
189
Issue
4
Year of publication
1993
Pages
405 - 410
Database
ISI
SICI code
0344-0338(1993)189:4<405:FCDAAP>2.0.ZU;2-3
Abstract
Fresh tumour tissue from 198 primary invasive breast carcinomas was an alysed by DNA flow cytometry. 108 tumours were non-diploid. A signific antly higher proportion of non-diploid tumours was found among node-po sitive patients, patients with oestrogen receptor negative tumours and among patients with ductal carcinomas. The survival of patients with diploid and non-diploid tumours was not significantly different (p = 0 .1). Totally, 145 tumours were analysed with respect to S-phase fracti on (SPF). The distribution of SPF was different in diploid and non-dip loid tumours. A low SPF group, defined as the lower SPF quartile (less -than-or-equal-to 4.6 % in diploid and less-than-or-equal-to 8.S % in non-diploid tumours), was associated with highly differentiated tumour s and oestrogen receptor positive tumours. Histological grading reveal ed a highly significant correlation to SPF. 57 % of ductal carcinomas grade I (8 out of 14), 30 % of ductal carcinomas grade II (20 out of 6 7) and S % of ductal carcinomas grade III (2 out of 37) had a low SPF. Patients within the low SPF group had a significantly longer survival than had patients within the high SPF group (p = 0.006). In a multiva riate analysis the SPF was found to be an additional prognostic factor next to node status and ER status.