M. Zahn et al., PURIFICATION AND N-TERMINAL SEQUENCE OF BETA-TRACE, A PROTEIN ABUNDANT IN HUMAN CEREBROSPINAL-FLUID, Neuroscience letters, 154(1-2), 1993, pp. 93-95
Beta-Trace, a protein that represents a major constituent of human cer
ebrospinal fluid with unknown function has been purified to apparent h
omogeneity by gel chromatography and electrophoresis. After sodium dod
ecylsulfate electrophoresis on polyacrylamide gels (SDS-PAGE) and West
ern blotting, the N-terminal sequence (28 amino acids) has been determ
ined. The high degree of identity with the corresponding sequences of
prostaglandin D synthetase from rat (68%) and human (93%) suggests a s
trong relationship if not identity with this enzyme. Thus, 30 years af
ter its discovery beta-trace might unravel as a well-known protein of
the prostaglandin metabolism.