FRUITING GENES OF SCHIZOPHYLLUM-COMMUNE ARE TRANSCRIPTIONALLY REGULATED

Fruiting genes in Schizophyllum commune are controlled by the mating-t ype genes and other regulatory genes. To examine whether differential accumulation of mRNAs for these fruiting genes is caused by transcript ional regulation, run-on transcription assaYs were performed with nucl ei isolated from cultures differing in expression of these genes. It c ould be shown that accumulation of mRNAs followed enhanced transcripti on of most of the fruiting genes. Transcription continued during subse quent decrease of these mRNAs. In young not-yet-fruiting dikaryons and non-fruiting monokaryons the fruiting genes appeared to be transcribe d at a low basal level. The GPD gene was used a control in these exper iments and was transcribed at all stages.