Gi. Denisov et Ai. Aleshin, DETERMINATION OF OPTIMUM CONDITIONS FOR OBTAINING KLEBSIELLA ANTIGENIC ERYTHROCYTE IMMUNOREAGENTS, Zurnal mikrobiologii, epidemiologii i immunobiologii, (2), 1993, pp. 102-106
The optimum conditions for obtaining sensitive K- and D-serovar-specif
ic Klebsiella antigenic erythrocyte diagnosticums have been establishe
d. The methods of choice are sensitization with lipopolysaccharide ant
igens at pH 5.0 and in a neutral medium at a temperature of 45-degrees
-C for an hour. The reagents thus obtained have proved to be specific
when tested in the passive hemagglutination test with antisera to Shig
ella, Salmonella and Escherichia.