CHLOROPLAST DNA DIVERSITY IN RASPBERRY

. The relationships among raspberry (Rubus spp.) clones were investiga ted using southern hybridization. Total DNA from 22 clones were digest ed with Bam HI and Eco RI and hybridized with two sequences from a Pst I tomato (Lycopersicon esculentum Mill.) chloroplast library. A total of 40 different restriction fragments were distinguished for the four enzyme probe combinations. These fragments distinguished seven groups of clones with members of each group having identical fragment patter ns. Clones with R. idaeus L. maternal ancestry were distinct from thos e with R. occidentalis L. or R. parvifolius L. ancestry. Differences w ere detected between R. idaeus vulgatus Arrhen. and R. idaeus strigosu s (Michx.). No commercial cultivars had chloroplast DNA patterns that were the same as an accession of the R. idaeus strigosus subspecies.