Granulocytes are known to exhibit positive chemotaxis towards erythroc
ytes destroyed by laser irradiation (necrotaxis). A computerized metho
d for visualizing intracellular calcium concentration in granulocytes
during necrotaxis has been developed. Successive bursts of fluorescenc
e were observed: 1-degrees) about 10 s after the creation of the necro
tactic source; 2-degrees) when the granulocyte touched the target.