THE EFFECT OF PH ON THE KINETICS OF PENICILLIN-G ACYLASE OBTAINED FROM A MUTANT OF ESCHERICHIA-COLI ATCC-11105

Authors
ERARSLAN A
Citation
A. Erarslan, THE EFFECT OF PH ON THE KINETICS OF PENICILLIN-G ACYLASE OBTAINED FROM A MUTANT OF ESCHERICHIA-COLI ATCC-11105, Process biochemistry, 28(5), 1993, pp. 319-324
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
28
Issue
5
Year of publication
1993
Pages
319 - 324
Database
ISI
SICI code
1359-5113(1993)28:5<319:TEOPOT>2.0.ZU;2-4
Abstract
The competitive inhibition of pH on the kinetics of penicillin G (Pen G) hydrolysis by penicillin G acylase (PGA) obtained from a mutant der ivative of Escherichia coli ATCC 11105 was investigated The effect of pH on the initial reaction rate was interpreted according to a mechani sm discussed by Tippon and Dixon. Molecular dissociation constants pK( B)E and pK(B)ES of PGA were calculated to be 9.783 and 10.851 respecti vely.