GLUCONEOGENESIS IS LESS ACTIVE IN BHE CDB RATS FED MENHADEN OIL THAN IN RATS FED BEEF TALLOW/

The effect of dietary fat on gluconeogenesis in BHE/cdb rats was studi ed. BHE/cdb male weanling rats were fed 1% corn oil plus 9% BT or MO u ntil they were 84 +/- 2 days of age. Glucose synthesis from 10mM lacta te, 10mM glycerol, 10mM alanine, or a 10:1mM mixture of lactate:pyruva te was determined in hepatocytes isolated from 48 hour starved rats. H epatocytes from BT fed rats synthesized 39% more glucose from lactate, 35% more from pyruvate, 44% more from alanine, 22% more from glycerol , and 51% more from the 10:1, lactate:pyruvate, than did cells from MO fed rats. In addition, hepatic tissue from 48 hour starved animals of both diet groups was analyzed for concentrations of pyruvate, lactate , malate, inorganic phosphate and the adenine nucleotides. No signific ant diet differences were observed in the concentrations of these meta bolites nor were there diet differences in the induction of phosphoeno lpyruvate carboxykinase (PEPCK) activity following food withdrawal. De spite a lack of a diet fat effect on the activity of PEPCK and on meta bolic precursors of glucose synthesis, gluconeogenesis was enhanced in the beef tallow fed rat.