Ae. Hiott et Rp. Phelps, EFFECTS OF INITIAL AGE AND SIZE ON SEX REVERSAL OF OREOCHROMIS-NILOTICUS FRY USING METHYLTESTOSTERONE, Aquaculture, 112(4), 1993, pp. 301-308
The influence of age and size of Oreochromis niloticus fry at the init
iation of hormone treatment on production of phenotypic male populatio
ns was investigated. A population of fry was divided into two lots. Th
e first (Age I) was graded into four size categories: 11 mm and less,
12-13 mm, 14-16 mm, and greater than 16 mm total length. The second lo
t (Age II) was held an additional 10 days then graded into the same si
ze categories. All groups were given methyltestosterone at 60 mg per k
g of feed for 28 days. Initial size was a more significant factor than
age in affecting the percentage of phenotypic males observed. The dur
ation of hormone treatment while fry were less than 18 mm length was s
ignificantly related to the percent males obtained, fish receiving les
s than 14 days of treatment had a lower percentage of males.