DEVELOPMENT OF THE PARTICLE INFLOW GUN

Authors
VAIN P KEEN N MURILLO J RATHUS C NEMES C FINER JJ
Citation
P. Vain et al., DEVELOPMENT OF THE PARTICLE INFLOW GUN, Plant cell, tissue and organ culture, 33(3), 1993, pp. 237-246
Citations number
16
Categorie Soggetti
Plant Sciences
Journal title
Plant cell, tissue and organ culture → ACNP
ISSN journal
01676857
Volume
33
Issue
3
Year of publication
1993
Pages
237 - 246
Database
ISI
SICI code
0167-6857(1993)33:3<237:DOTPIG>2.0.ZU;2-N
Abstract
A simple and inexpensive particle acceleration apparatus was designed for direct delivery of DNA to plant cells. The Particle Inflow Gun (PI G) is based on acceleration of DNA-coated tungsten particles directly in a helium stream. High levels of transient expression of the beta-gl ucuronidase gene were obtained following bombardment of embryogenic su spension cultures of maize and soybean, and leaf tissue of cowpea. Sta ble transformation of soybean and maize has also been obtained using t his bombardment apparatus.