The O-17 NMR spectra of cholesterol and 31 other steroidal alcohols, e
sters, ketones, and acids enriched by synthesis with O-17 from (H2O)-O
-17 have been observed under ordinary operating conditions, and correl
ations between O-17 chemical shift and structure have been adduced. Sp
ectra-structure correlations for these steroids are in conformity with
those previously adduced with simpler compounds by others.