Wt. Seo et al., IDENTIFICATION AND PRODUCTION OF FLAVONOIDS IN A CELL-SUSPENSION CULTURE OF SCUTELLARIA-BAICALENSIS G, Plant cell reports, 12(7-8), 1993, pp. 414-417
A high yielding cell line of Scutellaria baicalensis G. has successful
ly been developed to produce flavonoids. Major components of the flavo
noids were identified as baicalin and wogonin-7-O-glucuronic acid by a
series of instrumental analyses using UV, IR, MASS, and NMR. After 12
days in suspension culture, the cell growth reached 14 g(DW)/l, and b
aicalin and wogonin-7-O-glucuronic acid were obtained in concentration
s of 2.9 g/l and 1.07 g/l, respectively. The culture temperature was f
ound to be an important parameter for improving production yield of th
e flavonoids. The yield of baicalin was observed to increase to 4.2 g/
l by shifting the temperature from 30-degrees-C to 25-degrees-C after
72 h of suspension culture.