STUDIES IN SWINE ON INHERITANCE AND VARIATION IN EXPRESSION OF SMALL-INTESTINAL RECEPTORS MEDIATING ADHESION OF THE K88 ENTEROPATHOGENIC ESCHERICHIA-COLI VARIANTS
Zl. Hu et al., STUDIES IN SWINE ON INHERITANCE AND VARIATION IN EXPRESSION OF SMALL-INTESTINAL RECEPTORS MEDIATING ADHESION OF THE K88 ENTEROPATHOGENIC ESCHERICHIA-COLI VARIANTS, The Journal of heredity, 84(3), 1993, pp. 157-165
Small intestinal enterocyte preparations from 368 pigs were phenotyped
by an in vitro adhesion test using Six strains of K88 Escherichia (E.
) coli, each expressing one of three K88 fimbrial antigenic variants:
K88ab, K88ac, or K88ad. All pigs tested were classified into one of fo
ur adhesion brush border phenotyes: I (K88ab-, K88ac-, K88ad-); II (K8
8ab-, K88ac+, K88ad+); III(K88ab+, K88ac+, K88ad-); or IV (K88ab+, K88
ac+, K88ad+). The segregation and adhesion affinity data suggest that
there are two adhesion affinity receptors for K88ad+ E. coli: a high a
ffinity (adh) and a low affinity (adl) receptor. The high affinity rec
eptor cosegregates with receptors for K88ab and K88ac fimbrial antigen
s forming together the phenotype IV; the low affinity receptor is asso
ciated with the adhesion phenotype II, and its physiological expressio
n is terminated by 16 weeks of age. In contrast, the K88adH receptor i
s expressed during the entire life cycle. The presence of a mixed adhe
sion phenotype, K88adM, assumed to be determined by K88ab(-),ac(-),adl
(+)/K88ab(+),ac(+),adh(+) heterozygous genotype, is interpreted as an
indication that each of the two types of brush border adhesion for the
K88ad antigen is expressed on independent enterocytes.