QUANTITATIVE-ANALYSIS OF TEMAZEPAM IN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY - APPLICATION TO PHARMACOKINETIC STUDIES IN RATS

Authors
ESCORIAZA J DIOSVIEITEZ MC TROCONIZ IF RENEDO MJ FOS D
Citation
J. Escoriaza et al., QUANTITATIVE-ANALYSIS OF TEMAZEPAM IN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY - APPLICATION TO PHARMACOKINETIC STUDIES IN RATS, Chromatographia, 44(3-4), 1997, pp. 169-171
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
44
Issue
3-4
Year of publication
1997
Pages
169 - 171
Database
ISI
SICI code
0009-5893(1997)44:3-4<169:QOTIPB>2.0.ZU;2-A
Abstract
A sensitive method was developed for the determination of temazepam in plasma using capillary gas chromatography. After the extraction into dichloromethane-pentane (1:1), temazepam was quantitated as its O-trim ethylsilyl derivative on a capillary column with a Ni-63 electron capt ure detector using prazepam as internal standard. The detector respons e was found to be linear in the concentration range 0.031 to 8 mu g mL (-1). The detection limit was about 3.5 ng mL(-1). The intraday and in ter-day coefficients of variation were below 9%. The method was used t o determine the pharmacokinetic profile of temazepam in rats after int ravenous administration.