PREPARATIVE CHROMATOGRAPHY OF BIOMOLECULES

An overview of preparative chromatography is given with a description of both theoretical and practical aspects. Owing to the large-scale pr oduction of recombinant proteins in various hosts, the requirements fo r speed, recovery and purity are ever increasing. The introduction of new gels with higher stability, a better understanding of the adsorpti on process and significant improvements in equipment such as injectors , pumps, fractionation devices and valves have transformed chromatogra phy from an art to science and technology. The rules for scale-up are well understood (constant height, constant height-to-diameter ratio, d ynamic similarity) and theoretical solutions including computer progra ms are available to minimize experimental work.