NUCLEAR-MAGNETIC-RESONANCE (NMR) IN BIOCHEMICAL EXPLORATION OF SEMINAL PLASMA IN INFERTILE MEN

The purpose of this study was to investigate whether the measurement b y NMR of some biochemical markers such as glycerylphosphorylcholine (G PC), glycerylphosphorylethanolamine (GPE), citrate, and lactate in hum an seminal plasma may be able to differenciate spermatogenic failure f rom obstructive azoospermia. The peak area ratios citrate/lactate, bet ween normozoospermic and spermatogenic failure or obstructive azoosper mia groups were significantly different. We have also found significan t differences in GPE/GPC peak intensity ratio between spermatogenic fa ilure men and obstructive azoospermia patients (P < 0,001). This ratio appears to be a very important parameter to differenciate spermatogen ic failure from obstructive azoospermia. These results demonstrate the potential use of 1H NMR on human seminal plasma in male infertility e valuation ans such analysis may be used to elucidate the molecular bas is of the human pathologic seminal plasma disturbances.