IMPROVED METHOD OF TOTAL HISTONE ISOLATION FROM ARABIDOPSIS-THALIANA

Standard methods of isolation of chromatin histones from Arabidopsis t haliana yield strongly degraded proteins when applied to plants grown from seeds in axenic liquid media. For isolation of undegraded histone s from Arabidopsis grown in liquid media we used extraction with guani dine hydrochloride followed by selective binding of histones on BioRex 70 resin in the batch system. The quality of obtained proteins is con firmed by SDS polyacrylamide gel electrophoresis.