Shoot regenerations from hypocotyls and cotyledons of tomato (Lycopers
icon esculentum Mill.) was inhibited by NaCl (100 and 150 mM). Shoot f
resh and dry masses were also reduced. Addition of proline (100 mg dm(
-3)) counteracted the inhibitory effect of NaCl. SDS-PAGE analyses of
extracted proteins, revealed that in cultures grown in medium with 25
mM NaCl plus proline, extra polypeptides of M(r) 190, 58, 45 and 26 kD
a accumulated. As NaCl was increased in the medium a new protein of M(
r) 67 kDa also accumulated.