REGENERATION OF PLANTS FROM CALLUS-TISSUE OF DESMODIUM-AFFINE AND DESMODIUM-UNCINATUM

Plants were in vitro regenerated from leaf callus of Desmodium affine and D. uncinatum, Leaf explants were induced to form callus when asept ically cultured on Murashige and Skoog medium (MS) supplemented with 6 mg dm(-3) 6-benzylaminopurine (BAP) in combination with 1 mg dm(-3) n aphthaleneacetic acid (NAA). Regeneration of shoots was induced when c allus was cultured on MS medium supplemented with 6 mg dm(-3) BAP and 0.01 mg dm(-3) NAA. Roots regenerated in high frequency when different iated shoots were subcultured on MS medium supplemented only with 0.01 mg dm(-3) NAA. The regenerated plantlets were successfully grown in p ots. Calli from D. incanum failed to regenerate shoots.