LIPID HYDROPEROXIDE-RESISTANCE GENE IN SACCHAROMYCES-CEREVISIAE - UTILIZATION AS A SELECTABLE MARKER GENE FOR YEAST TRANSFORMATION

Two kinds of DNA fragments concerned with resistance against lipid hyd roperoxide were cloned from the yeast Saccharomyces cerevisiae. When t he recombinant plasmid carrying the DNA fragment was introduced into t he cells of S. cerevisiae which do not have an appropriate auxotrophic marker, the transformants could be selected by their resistance to t- butyl hydroperoxide. By Southern hybridization analysis, such transfor mants were found to contain the recombinant plasmid, suggesting that t he DNA fragment could be used as a selection marker for the transforma tion of S. cerevisiae.