Annatto (Bixa orellana L.) has become of increasing importance because
of the dyes, particularly bixin, produced in its seeds. Such dyes are
of great advantage as compared to synthetic ones. Since asexual propa
gation is more advantageous than sexual propagation it has stimulated
the study of, methods of vegetative propagation in annatto plants, inc
luding air layering. For the successfulness of this process, the subst
rate is a determing factor. This work had as objective to study the ef
fects, on the rooting of annatto air-layered branches, of substrates:
red sand (S), day (C), wood dust (W) and combinations among them. Four
teen plants were selected, each one representing a replication of the
randomized block design utilized. The rooting of the air-layered branc
hes was evaluated regarding rooting date and root number, fresh and dr
y weight, and volume. The substrates S, B and 1/2S + 1/2C were the mos
t effective, while the least effective were W and 1/2S + 1/2W. The oth
er evaluated substrates were of intermediate efficiency. There were po
sitive and significant correlations regarding leaf number and root num
ber, leaf number and root fresh weight, leaf number and root dry weigh
t, and leaf number and root volume.