My. Levy et al., EFFICACY EVALUATION OF A NOVEL SUBMICRON AMPHOTERICIN-B EMULSION IN MURINE CANDIDIASIS, Journal of medical and veterinary mycology, 31(3), 1993, pp. 207-218
A submicron amphotericin B (AmB) sterile emulsion with a mean droplet
size of approximately 100 nm was prepared. The emulsion was stable at
4-degrees-C over a period of 4 months. The acute toxicity results show
ed that the maximum tolerated dose of the AmB emulsion was 2.0 mg kg-1
as compared to 0.5 mg kg-1 for a commercial deoxycholate suspension,
Fungizone(R). Efficacy evaluations of submicron AmB emulsion compared
to Fungizone(R) were performed in a murine candidiasis model using eit
her a single or a multiple daily dose administration of 0-4 mg kg-1. S
urvival (100%) was observed up to 15-18 days post-infection in mice tr
eated with AmB emulsion or Fungizone(R), while all control non-treated
animals had died after 9 days in both single and multi-treatment expe
riments. Survival (50%) was obtained after 35 days in the single treat
ment, and 40 days in the multi-treatment, experiment with AmB emulsion
, while treatment with Fungizone(R) gave 50% survival after 15 and 20
days, respectively. The overall results indicate that in murine candid
iasis, treatment with AmB submicron emulsion was more effective than F
ungizone(R), suggesting a potential therapeutic application.