LIMITED PROTEOLYSIS AND SITE-DIRECTED MUTAGENESIS OF THE NF-KAPPA-B P50 DNA-BINDING SUBUNIT

Authors
SODEOKA M LARSON CJ CHEN L LANE WS VERDINE GL
Citation
M. Sodeoka et al., LIMITED PROTEOLYSIS AND SITE-DIRECTED MUTAGENESIS OF THE NF-KAPPA-B P50 DNA-BINDING SUBUNIT, Bioorganic & medicinal chemistry letters, 3(6), 1993, pp. 1095-1100
Citations number
12
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
3
Issue
6
Year of publication
1993
Pages
1095 - 1100
Database
ISI
SICI code
0960-894X(1993)3:6<1095:LPASMO>2.0.ZU;2-2
Abstract
Tryptic digestion was used to obtain an active 41 kDa fragment of NF-k appaB. The major proteolysis products were comprised of residues 2-364 (p50(2-364)), 2-362 (p50(2-362)) and 2-366 (p50(2-366)). By site-dire cted truncation mutagenesis, overproducers for p50(2-364) and p50(2-36 6) were constructed. These vectors direct high level expression of hom ogeneous, active protein.