Da. Cancilla et al., ISOLATION AND CHARACTERIZATION OF MICROTOX(R)-ACTIVE COMPONENTS FROM AIRCRAFT DE-ICING ANTI-ICING FLUIDS/, Environmental toxicology and chemistry, 16(3), 1997, pp. 430-434
The goal of this project was to isolate and identify individual compon
ents from aircraft de-icing/anti-icing fluids (ADAFs) through a toxici
ty-based bioassay analysis. A Microtox(R) bioassay fractionation schem
e was used to isolate a number of active fractions from ADAFs. Active
fractions were identified using multiple spectral techniques, includin
g nuclear magnetic resonance, gas chromatography-mass spectrometry, li
quid chromatography-mass spectrometry, and ultraviolet characterizatio
n, The primary Microtox-active fraction was shown to be a mixture of b
enzotriazole and tolyltriazoles, which are used as corrosion inhibitor
s in ADAF formulations. The identity of the compounds was confirmed th
rough spectral and Microtox-toxicity analysis and comparison of commer
cially available standards.