ISOLATION AND CHARACTERIZATION OF MICROTOX(R)-ACTIVE COMPONENTS FROM AIRCRAFT DE-ICING ANTI-ICING FLUIDS/

The goal of this project was to isolate and identify individual compon ents from aircraft de-icing/anti-icing fluids (ADAFs) through a toxici ty-based bioassay analysis. A Microtox(R) bioassay fractionation schem e was used to isolate a number of active fractions from ADAFs. Active fractions were identified using multiple spectral techniques, includin g nuclear magnetic resonance, gas chromatography-mass spectrometry, li quid chromatography-mass spectrometry, and ultraviolet characterizatio n, The primary Microtox-active fraction was shown to be a mixture of b enzotriazole and tolyltriazoles, which are used as corrosion inhibitor s in ADAF formulations. The identity of the compounds was confirmed th rough spectral and Microtox-toxicity analysis and comparison of commer cially available standards.