SYSTEMATIC OPTIMIZATION OF CAPILLARY ELECTROPHORETIC SEPARATIONS USING THE OVERLAPPING RESOLUTION MAPPING SCHEME

In this study, the use of a systematic optimization scheme for capilla ry electrophoretic separations is described. This scheme utilizes the overlapping resolution mapping procedure to predict the optimum separa tion conditions for the separation of two groups of compounds, a group of seven flavonoids and a group of eight sulfonamides. The chosen par ameters that affect the separation depend on the compounds in question . In the case of flavonoids, pH and SDS concentrations were chosen as the parameters for optimization, whereas in the case of sulfonamides, pH and beta-cyclodextrin concentrations were used. As few as nine expe riments are required to achieve optimum conditions for the separation of all peaks. To confirm the validity of the optimization procedure, a dditional experiments chosen from the predicted optimum conditions wer e performed. The results show that satisfactory separation could be ac hieved by using the optimum conditions predicted by the scheme. All fl avonoid and sulfonamide peaks were satisfactorily separated in about 3 0 and 8 min, respectively.