MICROENCAPSULATION SELECTION FOR ISOLATION OF YEAST MUTANTS WITH INCREASED SECRETION OF ASPERGILLUS-AWAMORI GLUCOAMYLASE

Authors
WITTRUP KD WEBER AL TSAI P
Citation
Kd. Wittrup et al., MICROENCAPSULATION SELECTION FOR ISOLATION OF YEAST MUTANTS WITH INCREASED SECRETION OF ASPERGILLUS-AWAMORI GLUCOAMYLASE, Biotechnology and bioengineering, 42(3), 1993, pp. 351-356
Citations number
33
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology and bioengineering → ACNP
ISSN journal
00063592
Volume
42
Issue
3
Year of publication
1993
Pages
351 - 356
Database
ISI
SICI code
0006-3592(1993)42:3<351:MSFIOY>2.0.ZU;2-S
Abstract
We have developed a microencapsulation selection method which allows t he rapid and quantitative screening of >10(6) yeast cells for enhanced secretion of Aspergillus awamori glucoamylase. The method provides a 400-fold single-pass enrichment for high-secreting mutants, and can be straightforwardly adapted for application to growth-based selection s chemes with other microorganisms and enzymes.