IMMUNOFLUORESCENCE STAINING OF RENAL BIOPSY SAMPLES IN PATIENTS WITH DIABETIC NEPHROPATHY IN NON-INSULIN-DEPENDENT DIABETES-MELLITUS USING MONOCLONAL-ANTIBODY TO REDUCED GLYCATED LYSINE

This is the first report on immunofluorescence staining of renal biops y samples in human diabetic nephropathy (DN) using monoclonal antibodi es to reduced glycated lysine. In order to detect the localization of glycated lysine in the mesangial matrix and/or the glomerular basement membrane (GBM), we examined immunofluorescence staining using antibod ies against reduced glycated lysine in the glomeruli of 16 patients wi th DN and ten age-matched patients with diffuse mesangial proliferativ e glomerulonephritis without IgA deposition (DPGN) as controls. In the early stage of DN, immunofluorescence microscopy revealed the presenc e of intense staining for reduced glycated lysine in the GEM as well a s in part of the tubular basement membrane, but not in the mesangial a rea. In contrast, immunofluorescence microscopy revealed less staining for glycated lysine in the GBM in the advanced stage of DN, and no re action with any part of the renal tissue in patients with DPGN. It was concluded that detection of reduced glycated lysine in GBM in the ear ly stage of DN might be associated with the initial pathogenesis of th is disease. (C) Elsevier Science Inc., 1996.