C. Riveromelian, SIMULTANEOUS DEMONSTRATION OF CENTRAL PROJECTIONS OF DIFFERENT PERIPHERAL-NERVES BY ANTI-CHOLERAGENOID IMMUNOGLOBULIN MARKERS, NeuroReport, 4(6), 1993, pp. 743-746
THIS study demonstrates a fluorescent antibody technique where immunog
lobulins (IgG) can be used as transganglionic tracers for the simultan
eous demonstration of different primary afferent nerve projections to
the spinal cord. Antiserum against choleragenoid (CTB) raised in goat
or rabbit was mixed with CTB to obtain a solution consisting of IgG bo
und to CTB. The mixtures of rabbit and goat IgG bound to CTB were subs
equently injected into the sciatic or femoral nerves. Following transg
anglionic transport, goat and rabbit IgG staining were demonstrated si
multaneously in different areas of the L4 dorsal root ganglion, in the
spinal cord and in the brain stem, using an immunocytochemical techni
que. When only goat anti-CTB antibodies or only CTB were injected into
the sciatic nerve, no goat IgG immunoreactivity could be detected in
the spinal cord.