TRANSCRIPTIONAL ANALYSIS OF THE GAP-PGK-TPI-PPC GENE-CLUSTER OF CORYNEBACTERIUM-GLUTAMICUM

The transcriptional organization of the Corynebacterium glutamicum gap -pgk-tpi-ppc gene cluster, encoding glyceraldehyde-3-phosphate dehydro genase, 3-phosphoglycerate kinase, triosephosphate isomerase, and phos phoenolpyruvate carboxylase, was investigated by Northern (RNA) blot a nd primer extension analyses. Four transcripts corresponding to gap, t o gap-pgk-tpi, to pgk-tpi, and to pgk-tpi-ppc were identified. The res pective transcriptional initiation sites in front of gap and pgk were located, and, from the analysis of DNA sequences upstream of these and of previously determined transcriptional start sites, common structur es which may be important for promoter function in C. glutamicum are d iscussed.