INHIBITION OF THE MITOCHONDRIAL CALCIUM UNIPORTER BY ANTIBODIES AGAINST A 40-KDA GLYCOPROTEIN(T)

Authors
SARIS NEL SIROTA TV VIRTANEN I NIVA K PENTTILA T DOLGACHOVA LP MIRONOVA GD
Citation
Nel. Saris et al., INHIBITION OF THE MITOCHONDRIAL CALCIUM UNIPORTER BY ANTIBODIES AGAINST A 40-KDA GLYCOPROTEIN(T), Journal of bioenergetics and biomembranes, 25(3), 1993, pp. 307-312
Citations number
31
Categorie Soggetti
Biophysics,"Cytology & Histology
Journal title
Journal of bioenergetics and biomembranes → ACNP
ISSN journal
0145479X
Volume
25
Issue
3
Year of publication
1993
Pages
307 - 312
Database
ISI
SICI code
0145-479X(1993)25:3<307:IOTMCU>2.0.ZU;2-E
Abstract
Polyclonal rabbit antibodies against a Ca2+-binding mitochondrial glyc oprotein were found to inhibit the uniporter-mediated transport of Ca2 + in mitoplasts prepared from rat liver mitochondria. Spermine, a modu lator of the uniporter, decreased the inhibition. This glycoprotein of M(r) 40,000, isolated from beef heart mitochondria and earlier shown to form Ca2+-conducting channels in black-lipid membranes, thus is a g ood candidate for being a component of the uniporter. Antibody-IgG was found to specifically bind to mitochondria in human fibroblasts.