PRECOLUMN DERIVATIZATION AND ITS OPTIMUM CONDITIONS FOR QUANTITATIVE-DETERMINATION OF URINARY HYDROXYLYSINE GLYCOSIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A method has been developed for rapid analysis of the urinary hydroxyl ysine glycosides [glucosyl-galactosyl- hydroxylysine (GGH) and galacto syl-hydroxylysine (GH)] as collagen metabolites. The glycosides were f luorometrically derivatized with 5-dimethylaminonaphthalene-1-sulfonyl chloride, and then subjected to reversed-phase high-performance liqui d chromatography using a gradient system. Suitable standard hydroxylys ine glycosides which were not diastereoisomers were prepared from huma n urine. Urinary GGH and GH were analyzed within 1 h, including column washing. This method was applied to the urine samples of 68 healthy p ersons of different ages.