R. Ihara et al., DUAL-TIMES DETECTION SYSTEM FOR ONLINE ASSAY OF ENZYMATIC-ACTIVITY BASED ON VALVE-SWITCHING TECHNIQUE USING SIZE-EXCLUSION CHROMATOGRAPHY, Chemistry Letters, (6), 1993, pp. 1013-1016
A dual-times detection system in size exclusion chromatography was dev
eloped for carboxypeptidase A assay. In the system the enzymatic activ
ity was detected 2 times by circulating the sample solution (mobile ph
ase) in a reaction tube through a fluorescence detector.