ADHERENCE IN COMBINATION WITH LIPOPOLYSACCHARIDE, TUMOR-NECROSIS-FACTOR OR INTERLEUKIN-1-BETA POTENTIATES THE INDUCTION OF MONOCYTE-DERIVEDINTERLEUKIN-8

In this study we demonstrate that adherence of peripheral blood mononu clear cells (PBMC) to either plastic or extracellular matrix, such as collagen type I or fibronectin, is a significant stimulus for the indu ction of both interleukin-8 (IL-8) mRNA and antigen. In addition, adhe rence of PBMC in the presence of lipopolysaccharide, interleukin-1-bet a, or tumor necrosis factor-alpha greatly enhances transcription/trans lation of IL-8 from these leukocytes. Our findings demonstrate that PB MC adherent to either plastic or physiological surfaces in combination with an inflammatory agonist is both a potent and efficacious signal for the expression and production of the neutrophil chemotactic/activa ting cytokine, IL-8.