MICROENCAPSULATION OF SILICONE OILS WITHIN POLYAMIDE POLYETHYLENIMINEMEMBRANES AS OXYGEN CARRIERS FOR BIOREACTOR OXYGENATION

Citation
D. Poncelet et al., MICROENCAPSULATION OF SILICONE OILS WITHIN POLYAMIDE POLYETHYLENIMINEMEMBRANES AS OXYGEN CARRIERS FOR BIOREACTOR OXYGENATION, Journal of chemical technology and biotechnology, 57(3), 1993, pp. 253-263
Citations number
29
Categorie Soggetti
Engineering, Chemical",Chemistry,"Biothechnology & Applied Migrobiology
ISSN journal
02682575
Volume
57
Issue
3
Year of publication
1993
Pages
253 - 263
Database
ISI
SICI code
0268-2575(1993)57:3<253:MOSOWP>2.0.ZU;2-7
Abstract
Silicone oils were microencapsulated within polyamide (nylon) membrane s cross-linked with polyethylenimine. Solubility of oxygen within the silicones, whether encapsulated or not, was approximately 6 mmol dm-3, representing solubilities approximately 20-fold higher than that of o xygen in water. The response time of oxygen transfer from the microenc apsulated oxygen carrier was less than 2.5 s; a more precise measureme nt was limited by the response time characteristics of the oxygen prob e. Assuming that the bioreactor volume consists of 10-20 % (v/v) micro encapsulated silicone oil, this represents an enhancement of the oxyge n reservoir by a factor ranging from 4 to 7 and improvement in oxygen transfer rates greater than 15-fold due to the greatly increased speci fic surface area in comparison to conventional bubble aeration. Circul ation of oxygenated silicone oils encapsulated within inert polymeric membranes may provide an efficient alternative to bioreactor oxygenati on with shear sensitive cell systems, or in fermentations with high ox ygen requirements.