INVITRO AND INVIVO MEASUREMENT OF CELL-MEDIATED-IMMUNITY IN PATIENTS WITH HIV-1 INFECTION

Citation
Jcc. Borleffs et al., INVITRO AND INVIVO MEASUREMENT OF CELL-MEDIATED-IMMUNITY IN PATIENTS WITH HIV-1 INFECTION, Scandinavian journal of immunology, 37(6), 1993, pp. 634-636
Citations number
10
Categorie Soggetti
Immunology
ISSN journal
03009475
Volume
37
Issue
6
Year of publication
1993
Pages
634 - 636
Database
ISI
SICI code
0300-9475(1993)37:6<634:IAIMOC>2.0.ZU;2-G
Abstract
We studied the usefulness of the in vitro lymphoproliferation assay an d the in vivo skin test in HIV-1-infected patients by using Clostridiu m tetani and tuberculin as testing antigens. Moreover, the relationshi p between data obtained from both assays was studied. In 56 HIV-infect ed patients not receiving antiretroviral therapy CD4+ cell counting wa s performed. In addition, in vitro (lymphocyte proliferation assay) an d in vivo (delayed type hypersensitivity skin test) measuring of the i mmune status was done using C. tetani and tuberculin as testing antige ns. When using C. tetani a significant correlation between the results of both tests and the CD4+ cell count was found. In contrast to earli er reports from African countries, in vivo skin testing using tubercul in did not yield clinically significant information on the degree of i mmunodeficiency. We explain our findings by the fact that health care policy in The Netherlands encompasses vaccination with C. tetani, whic h enables the application of C. tetani as testing antigen for measurin g immune function both in vitro and in vivo.