INDUCTION OF PHOSPHATIDYLINOSITOL-LINKED FC-GAMMA RECEPTOR-III EXPRESSION ON AN EOSINOPHILIC CELL-LINE, EOL-1, BY DIBUTYRYL-CYCLIC-AMP AND INTERFERON-GAMMA

The expression of Fcgamma receptor III (FcgammaRIII) on a human eosino philic leukemia cell line, EoL-1, was examined and compared with its e xpression on normal blood eosinophils. Surface FcgammaRIII expression on EoL-1 cells could be induced in vitro with a combination of dibutyr yl cAMP (dbcAMP) and gamma-interferon (IFN-gamma), but not with IFN-ga mma or dbcAMP alone. Pretreatment of EoL-1 cells with dbcAMP induced E oL-1 cells to express FcgammaRIII when stimulated with IFN-gamma, but EoL-1 cells pretreated with IFN-gamma and then stimulated with dbcAMP failed to express FcgammaRIII. Cyclic AMP was shown to play a role in the effect of dbcAMP. Both the treatment with phosphatidyl-inositol-sp ecific phospholipase C (PI-PLC) and the restriction enzyme digestion o f FcgammaRIII cDNA showed that the FcgammaRIII on EoL-1 cells was a ph osphatidylinositol-linked form. On the other hand, freshly isolated bl ood eosinophils constitutively expressed few, if any, FcgammaRIII, and IFN-gamma induced FcgammaRIII expression on them in vitro. Dibutyryl cAMP did not induce FcgammaRIII expression and even suppressed the IFN -gamma-induced FcgammaRIII expression on normal eosinophils. The EoL-1 cell line appears to be a useful in vitro model for the expression an d function of the phosphatidylinositol-linked form of FcgammaRIII on e osinophils.