Pulses of high voltage electric fields (PEF) are potentially a most im
portant cold pasteurization/sterilization food preservation technique
to replace or partially substitute for thermal processes. Du ring the
PEF process, lysis of microorganisms is caused by irreversible structu
ral changes in the membranes, leading to pore formation and destructio
n of the semipermeable barrier of the membrane. Theories explaining el
ectroporation of the cell membrane and applications of the nonthermal
PEF process are reviewed in this paper.