SELECTION OF AN ANTI-IGF-1 FAB FROM A FAB PHAGE LIBRARY CREATED BY MUTAGENESIS OF MULTIPLE CDR LOOPS

Authors
GARRARD LJ HENNER DJ
Citation
Lj. Garrard et Dj. Henner, SELECTION OF AN ANTI-IGF-1 FAB FROM A FAB PHAGE LIBRARY CREATED BY MUTAGENESIS OF MULTIPLE CDR LOOPS, Gene, 128(1), 1993, pp. 103-109
Citations number
26
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
128
Issue
1
Year of publication
1993
Pages
103 - 109
Database
ISI
SICI code
0378-1119(1993)128:1<103:SOAAFF>2.0.ZU;2-0
Abstract
Diverse Fab libraries containing 2-3 x 10(8) members were generated by randomizing amino acid residues within four of the six complementarit y determining regions of a humanized version of an anti-HER-2 Ab (hu4D 5). These libraries were subsequently displayed on the surface of the filamentous bacteriophage M13 and selected for binding to three protei ns: CD4, insulin-like growth factor 1 (IGF-1), and tissue plasminogen activator. An Fab-bacteriophage was isolated that showed specific bind ing to IGF-1. The affinity of this Fab was determined to be 3.5 muM.