INACTIVATION OF SIMIAN ROTAVIRUS SA-11 BY HYDROGEN-PEROXIDE

The kinetics and extent of the simian rotavirus SA 11 (RV) inactivatio n by low concentrations of hydrogen peroxide (HP) were determined. The curves illustrating the kinetics of crude virus inactivation have ''t ailing off''. The inactivation of the purified virus runs according to the first-order kinetics. Data indicated, that the purified RV was mo re sensitive to HP comparing to the crude virus. The purified RV was r elatively sensitive to 0.5-1.0% HP. The nonpurified RV was considerabl y more resistant. Only HP concentrations of 3% and higher were efficie nt. Virus sensitivity to the disinfectant strongly depends on pH of a medium in which it is suspended. The lowest inactivation rate occurs a t pH 7.4, the highest at pH 3.0. The strong temperature dependance of the virus inactivation rate by HP has been noticed. However no changes of the virus capsid after heating at 40-degrees-C able to facilitate the HP action at 0-degrees-C were observed. The HP action on virus par ticles at low temperature does not make them more thermolabile, when t he temperature shifts from 0-degrees-C to 40-degrees-C. So, the temper ature dependence of inactivation rate is probably determined only by t he reaction rate between a disinfectant and virus particles as targets . The horse-radish peroxidase in concentration 1-5-10 mg/ml considerab ly increased the virucidal effect of low HP concentrations. We think t hat mixtures of low HP concentrations with peroxidase are less disadva ntageous than higher HP concentrations showing the same virucidal effi ciency.