MODELING OF THE RETENTION BEHAVIOR OF SOLUTES IN MICELLAR LIQUID-CHROMATOGRAPHY WITH ORGANIC MODIFIERS

Most of the reported procedures for the determination of compounds by micellar liquid chromatography make use of micellar mobile phases cont aining an alcohol. The retention of a solute in a purely micellar elue nt has been adequately described by the linear equation 1/k' vs. micel le concentration. This equation seems also to be valid for mobile phas es with the same alcohol concentration and varying micelle concentrati ons. A model to describe the retention behaviour of solutes in any mob ile phase of surfactant and alcohol is proposed, which makes use of th e elution data in five mobile phases of surfactant with different amou nts of alcohol. A function of the type 1/k' = Amu + Bphi + Cmuphi + D, where mu and phi are surfactant and alcohol concentration, respective ly, proved to be satisfactory for different solutes (catecholamines, a mino acids, phenols and other aromatic compounds).