C. Puttikhunt et al., DISTRIBUTION IN THE GENUS STREPTOMYCES OF A HOMOLOG TO NUSG, A GENE ENCODING A TRANSCRIPTIONAL ANTITERMINATOR, FEMS microbiology letters, 110(2), 1993, pp. 243-248
The presence of the vbrA gene encoding the transcriptional antitermina
tor NusG equivalent protein of Streptomyces virginiae was tested for i
n 73 Streptomyces species by Southern hybridization. Fifty-five strain
s (75%) including S. griseus, S. lividans TK-21 and S. coelicolor A3(2
) showed clear hybridization signals, indicating wide distribution of
vbrA or vbrA homologs in Streptomyces species. With hybridization patt
erns against 3 different probes, i.e., probes covering vbrA alone, the
downstream gene rplK alone, and both vbrA-rplK, the 55 strains were c
lassified into 4 groups. In the groups I, II and III (total 50 strains
) vbrA was found to be adjacent to rplK, indicating that the gene arra
ngement vbrA-rplK is common in Streptomyces and that these genes may c
onstitute a part of gene cluster encoding several components of the tr
anscription and translation apparatus, as in Escherichia coli.