Cm. Bianchihall et al., DIVERSITY OF SEED STORAGE PROTEIN-PATTERNS IN WILD PEANUT (ARACHIS, FABACEAE) SPECIES, Plant systematics and evolution, 186(1-2), 1993, pp. 1-15
55 accessions of wild peanuts (Arachis spp.) introduced from South Ame
rica were analyzed for seed storage protein composition using SDS-PAGE
electrophoresis. The objectives of the study were to evaluate variabi
lity within sect. Arachis and to classify taxa based on protein compos
ition. 25 different band positions were resolved. Individual accession
s had 11 to 18 bands which included the conarachin region (MW > 50 kD)
, two to five bands in the acidic arachin region (MW 38-49.9 kD), thre
e to seven in the intermediate MW region (23 to 37.9 kD), two to five
bands in the basic arachin region (18-22.9 kD), and one to three bands
in the low MW protein region (14-17.9 kD). These data were utilized i
n a principal coordinate analysis based on the matrix of genetic dista
nces between all pairs of the 55 accessions. Several groups of accessi
ons conformed to expected species classification including A. batizoco
i, A. stenosperma, and A. monticola; while A. duranensis, A. cardenasi
i, A. helodes, and A. correntina did not form good groups. The study s
howed that great diversity exists for protein profiles and seed storag
e proteins have potential for aiding species classification and for se
rving as markers for interspecific hybridization studies.